Systems biology of liver cancer:
an integrative genomic-epigenomic approach (MODHEP)
The concept at the heart of MODHEP is that the pathologic behaviour of cancer cells is controlled by an abnormally reprogrammed genome: understanding cancer requires that the molecular basis for genome reprogramming be unraveled and described within an integrated model. To this aim, we are producing an integrated analysis of the nuclear changes occurring in two complementary multi-stage models of liver cancer. A coordinated, multi-faceted and quantitative series of datasets will be generated by the different labs in the consortium, forming a complete description of the genetic, epigenetic and nuclear organizational aberrations occurring both at pre-tumoral and tumoral stages of the disease. A combination of innovative systems biology-based approaches will then bring together the analysis of functional pathways with computational models of genomic, epigenomic and three-dimensional nuclear organization. [ read all ]
Concept of MODHEP
- generate genome-wide datasets from homogeneous cell populations in the experimental mouse models, and in part on clinical samples;
- generate a systems-based model network that integrates all the data acquired experimentally;
- assess the validity of the main features of this model network in the human disease.